rabbit total gsk3ß cell signaling  (Cell Signaling Technology Inc)

Journal: NPJ Precision Oncology

Article Title: IL-11/IL-11R signal inhibition by 9MW3811 remodels immune tumor microenvironment and enhances anti-tumor efficacy of PD-1 blockade

doi: 10.1038/s41698-025-00913-w

Figure Lengend Snippet: A Binding activity of 9MW3811 with recombinant IL-11 from different species. B Binding activity of 9MW3811 with human IL-11, compared with X203, a reported anti-IL-11 neutralizing antibody. C Blocking activity of 9MW3811 against the formation of IL-11/IL-11Rα/gp130 protein complex, compared with X203. D Schematic representation of JAK/STAT3 signaling downstream of IL-11. E JAK/STAT3 signaling transduction inhibitions by 9MW3811 and X203 were performed by luciferase reporter gene assay, using IL-11Rα/STAT3-luc HEK293 cell line. F The inhibition of pSTAT3 stimulated by human IL-11 in HGC-27 tumor cells was detected by immunoblotting assay using Simple Western-Jess. Data is shown as mean± SD.

Article Snippet: The following primary and secondary antibodies were used: Total-STAT3 (12640, CST), phospho-STAT3 (9131, CST), vinculin (13901, CST), Total-ERK (4695, CST), phospho-ERK (80031-1-RR, Proteintech), β-Actin (4970, CST), Anti-Rabbit Detection Module (DM001, ProteinSimple).

Techniques: Binding Assay, Activity Assay, Recombinant, Blocking Assay, Transduction, Luciferase, Reporter Gene Assay, Inhibition, Western Blot, Simple Western